目的 建立同时测定肉桂药材中4种挥发油类成分肉桂醇、肉桂酸、桂皮醛、2-甲氧基肉桂醛含量的一测多评法,为制定肉桂药材的质量标准提供科学依据。方法 以桂皮醛为内参物,分别建立其与肉桂醇、肉桂酸、2-甲氧基肉桂醛的相对校正因子,并进行含量计算,实现一测多评;同时采用外标法测定肉桂药材中 4 种挥发油类成分的含量,比较一测多评法计算值与外标法实测值的差异,验证其在含量测定中的可行性和准确性。结果 建立的相对校正因子重现性良好,桂皮醛与肉桂醇、肉桂酸、2-甲氧基肉桂醛的相对校正因子分别为0.675、0.606和1.935,其RSD分别为0.529%、0.373%和0.759%;肉桂药材采用一测多评法计算的含量与外标法实测值没有显著性差异。结论 利用一测多评法可实现对肉桂药材中桂皮醛等4种挥发油类成分的含量测定,该方法简便可行、重复性好,可应用于肉桂药材中挥发油类成分的质量评价。建议提高肉桂药材中桂皮醛含量不低于2.5%,建议制定肉桂药材中肉桂醇、肉桂酸和2-甲氧基肉桂醛的总量不得少于0.2%。
Abstract
OBJECTIVE To establish a method of quantitative analysis of multi-components by single marker (QAMS) for determining four essential oils (cinnamaldehyde, cinnamyl alcohol, cinnamic acid, 2-methoxy cinnamaldehyde) in Cinnamomum cassia, and provide the experimental base for establishing the quality standard of Cinnamomum cassia. METHODS Cinnamaldehyde was used as the internal reference standard, and the relative correction factors (RCF) of cinnamyl alcohol, cinnamic acid, and 2-methoxy cinnamaldehyde in Cinnamomum cassia were calculated. The contents of the four components were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of the quantitative results of both methods. RESULTS The RCFs had good reproducibility, relative correction factor 0.673, 0.605 and 1.943, with RSDs of 0.529%, 0.373%, and 0.759%, respectively. No significant differences were found in the quantitative analysis results of cinnamyl alcohol, cinnamic acid, 2-methoxy cinnamaldehyde by using RCF and ESM. CONCLUSION In the absence of reference substance, the content determination of the four essential oils in Cinnamomum cassia can be realized by QAMS, and this method can be used in the multi-index evaluation of Cinnamomum cassia essential oil constituents. It is suggested that the standard for cinnamaldehyde content be increased to 2.5%, and the contents of total cinnamyl alcohol, cinnamic acid and 2-methoxy cinnamaldehyde be not less than 0.2%.
关键词
一测多评法 /
相对校正因子 /
肉桂 /
桂皮醛 /
外标法
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Key words
quantitative analysis of multi-components by single marker /
relative correction factor /
Cinnamomum cassia Presl /
cinnamaldehyde /
external standard method
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中图分类号：
R284
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脚注
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基金
国家新兴产业重大工程包中药标准化项目资助
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